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OPS DIAGNOSTICS zirconium bead prefilled tubes
Zirconium Bead Prefilled Tubes, supplied by OPS DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zirconium+beads+prefilled+tubes/zirconium+beads+prefilled+tubes/pm38010230-95-7-8
Average 90 stars, based on 1 article reviews
zirconium bead prefilled tubes - by Bioz Stars, 2026-10
90/100 stars

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Isolation:

Article Title: The vitamin B 3 analogue nicotinamide riboside has only very minor effects on reducing muscle damage in mdx mice
Article Snippet: Extracted sample was applied to a C18 HPLC column connected with a Shimadzu 20AD HPLC system (Shimadzu; Tokyo, Japan) for the separation and quantified with a Sciex AI5000 mass spectrometer (Sciex; Framingham, MA, USA). .. Protein was isolated from muscle samples by homogenizing the muscles in 1.4 mm Zirconium Beads prefilled tubes (OPS Diagnostics; Lebanon, USA), containing 100 mM Tris-HCl (pH 6.8) – 20% (w/v) sodium dodecyl sulphate (SDS), using a MagNA Lyser (Roche Diagnostics; Woerden, The Netherlands). .. Concentrations were measured using a Pierce bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific) according to manufacturer’s instructions.

Article Title: Simvastatin Treatment Does Not Ameliorate Muscle Pathophysiology in a Mouse Model for Duchenne Muscular Dystrophy
Article Snippet: Cq values were determined via regression and normalized to the geometric mean of CDC40 and Fbxo38 (taken from the geNormPLUS set, Primerdesign, United Kingdom). .. At LUMC, muscle sections were collected in 1.4 mm Zirconium Beads prefilled tubes (OPS Diagnostics, Lebanon, USA) and disrupted in TRIsure isolation reagent (GCBioetech, the Netherlands) using a MagNA Lyser (Roche Diagnostics, the Netherlands). .. Total RNA was isolated and cleaned up by applying a NucleoSpin RNA II kit (Macherey-Nagel, Germany) according to the manufacturer’s instruction. cDNA was synthesised from 0.5μg of total RNA using random N6 primers (Thermo fisher scientific) and Bioscript enzyme (GCBiotech, the Netherlands) according to the manufacturer’s instructions. qPCR was performed using SensiMix reagents (GCBiotech) and the LightCycler 480 (Roche), using a program consisting of 45 cycles of 95°C (10 s), 58°C (30 s) and 72°C (20 s).

Article Title: Simvastatin Treatment Does Not Ameliorate Muscle Pathophysiology in a Mouse Model for Duchenne Muscular Dystrophy
Article Snippet: .. Cq values were determined via regression and440 normalized to the geometric mean of CDC40 and441 Fbxo38 (taken from the geNormPLUS set, Primerde-442 sign, United Kingdom).443 At LUMC, muscle sections were collected in 1.4444 mm Zirconium Beads prefilled tubes (OPS Diag-445 nostics, Lebanon, USA) and disrupted in TRIsure446 isolation reagent (GCBioetech, the Netherlands)447 using a MagNA Lyser (Roche Diagnostics, the448 Netherlands). .. Total RNA was isolated and cleaned449 up by applying a NucleoSpin RNA II kit (Macherey-450 Nagel, Germany) according to the manufacturer’s451 instruction. cDNA was synthesised from 0.5 g of452 total RNA using random N6 primers (Thermo fisher453 scientific) and Bioscript enzyme (GCBiotech, the454 Netherlands) according to the manufacturer’s instruc-455 tions. qPCR was performed using SensiMix reagents456 (GCBiotech) and the LightCycler 480 (Roche), using457 a program consisting of 45 cycles of 95◦C (10 s),458 58◦C (30 s) and 72◦C (20 s).

Article Title: Voluntary exercise improves muscle function and does not exacerbate muscle and heart pathology in aged Duchenne muscular dystrophy mice.
Article Snippet: .. 2.8 RNA isolation and gene expression analysis Diaphragm muscles were placed in 1.4 mm Zirconium Beads prefilled tubes (OPS Diagnostics, Lebanon, USA) and homogenized in TRIsure isolation reagent (Bioline, London, United Kingdom) using a MagNA Lyser (Roche Diagnostics). .. Total RNA was isolated using the TRIsure isolation method and purified by applying a NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's instruction.

Article Title: Cross-sectional study into age-related pathology of mouse models for limb girdle muscular dystrophy types 2D and 2F.
Article Snippet: .. Muscle sections were collected in 1.4 mm Zirconium Beads prefilled tubes (OPS Diagnostics, Lebanon, USA) and disrupted in TRIsure isolation reagent (GCBioetech, Alphen aan den Rijn, the Netherlands) using a MagNA Lyser (Roche Diagnostics). .. Total RNA was isolated and cleaned up by applying a NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instruction. cDNA was synthesized from 0.3-0.5 μg of total RNA (depending on the muscle type but kept constant between comparisons of different genotypes and ages of the same muscle) using random N6 primers (Thermo fisher scientific) and Bioscript enzyme (GCBiotech, Alphen aan den Rijn, the Netherlands) according to the manufacturer’s instructions.

Article Title: Efficient Downregulation of Alk4 in Skeletal Muscle After Systemic Treatment with Conjugated siRNAs in a Mouse Model for Duchenne Muscular Dystrophy
Article Snippet: .. Muscles (tibialis anterior, gastrocnemius, diaphragm, and heart) and liver were isolated and frozen in zirconium beads prefilled tubes (1.4 mm; OPS Diagnostics) in liquid nitrogen and stored at −80°C. ..

Muscles:

Article Title: The vitamin B 3 analogue nicotinamide riboside has only very minor effects on reducing muscle damage in mdx mice
Article Snippet: Extracted sample was applied to a C18 HPLC column connected with a Shimadzu 20AD HPLC system (Shimadzu; Tokyo, Japan) for the separation and quantified with a Sciex AI5000 mass spectrometer (Sciex; Framingham, MA, USA). .. Protein was isolated from muscle samples by homogenizing the muscles in 1.4 mm Zirconium Beads prefilled tubes (OPS Diagnostics; Lebanon, USA), containing 100 mM Tris-HCl (pH 6.8) – 20% (w/v) sodium dodecyl sulphate (SDS), using a MagNA Lyser (Roche Diagnostics; Woerden, The Netherlands). .. Concentrations were measured using a Pierce bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific) according to manufacturer’s instructions.

Article Title: Voluntary exercise improves muscle function and does not exacerbate muscle and heart pathology in aged Duchenne muscular dystrophy mice.
Article Snippet: .. 2.8 RNA isolation and gene expression analysis Diaphragm muscles were placed in 1.4 mm Zirconium Beads prefilled tubes (OPS Diagnostics, Lebanon, USA) and homogenized in TRIsure isolation reagent (Bioline, London, United Kingdom) using a MagNA Lyser (Roche Diagnostics). .. Total RNA was isolated using the TRIsure isolation method and purified by applying a NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's instruction.

Article Title: Simvastatin Treatment Does Not Ameliorate Muscle Pathophysiology in a Mouse Model for Duchenne Muscular Dystrophy
Article Snippet: .. At LUMC, TA muscles were homogenised in buffer containing 100 mM Tris-HCl (pH 6.8)- 20% (w/v) sodium dodecyl sulfate (SDS) in 1.4 mm Zirconium Beads prefilled Tubes (OPS Diagnostics LLC, NJ, United States), using a MagNaLyzer (Roche). .. Protein concentrations were determined using a Pierce bicinchoninic acid protein assay kit (Thermo Fisher Scientific, MA, United States) according to manufacturer’s instructions.

Article Title: Efficient Downregulation of Alk4 in Skeletal Muscle After Systemic Treatment with Conjugated siRNAs in a Mouse Model for Duchenne Muscular Dystrophy
Article Snippet: .. Muscles (tibialis anterior, gastrocnemius, diaphragm, and heart) and liver were isolated and frozen in zirconium beads prefilled tubes (1.4 mm; OPS Diagnostics) in liquid nitrogen and stored at −80°C. ..

other:

Article Title: Simvastatin Treatment Does Not Ameliorate Muscle Pathophysiology in a Mouse Model for Duchenne Muscular Dystrophy
Article Snippet: 498 Protein analysis 499 At LUMC, TA muscles were homogenised in 500 buffer containing 100 mM Tris-HCl (pH 6.8)- 20% 501 (w/v) sodium dodecyl sulfate (SDS) in 1.4 mm Zirco- 502 nium Beads prefilled Tubes (OPS Diagnostics LLC, 503 NJ, United States), using a MagNaLyzer (Roche).

Gene Expression:

Article Title: Voluntary exercise improves muscle function and does not exacerbate muscle and heart pathology in aged Duchenne muscular dystrophy mice.
Article Snippet: .. 2.8 RNA isolation and gene expression analysis Diaphragm muscles were placed in 1.4 mm Zirconium Beads prefilled tubes (OPS Diagnostics, Lebanon, USA) and homogenized in TRIsure isolation reagent (Bioline, London, United Kingdom) using a MagNA Lyser (Roche Diagnostics). .. Total RNA was isolated using the TRIsure isolation method and purified by applying a NucleoSpin RNA II kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's instruction.



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